Articles
All articles | Recent articles
Synthesis and in vitro Bioactivity Mechanism of Synthetic α-wollastonite and β-wollastonite Bioceramics
R. Morsy, R. Abuelkhair, T. Elnimr
Biophysics Lab, Physics Department, Faculty of Science. Tanta University, Tanta 31527, Egypt
received June 23, 2015, received in revised form August 26, 2015, accepted September 30, 2015
Vol. 7, No. 1, Pages 65-70 DOI: 10.4416/JCST2015-00028
Abstract
Synthetic wollastonite ceramics have received great attention as promising bioceramics for enhancing the properties of bone substitutes. However, uncertainty remains about the differences in bioactivity between its phases, that is and , and about the mechanism for inducing bone-like hydroxyapatite (b-HAp). In the present study, and ceramics were prepared with the co-precipitation method. For the in vitro bioactivity test, and powders were soaked in simulated body fluid (SBF) solution for 1, 2, 3, 4, 10 days. Synthetic , and induced b-HAp particles were characterized by means of XRD, FT-IR, SEM and electron spin resonance (ESR). The results showed significant differences in the bioactivity between the and samples; the showed a significant increase in the rate of formation of b-HAp compared to that of . ESR data showed a high sensitivity to study the formation process of b-HAp, and confirmed the role of defects in bioactivity mechanism. The bioactivity mechanism was observed to involve a dissolution process of the wollastonite and formation process of the b-HAp. Therefore, these results describe the in vitro bioactivity differences between and powders, emphasizing the importance of controlling their bioactivity parameter for developing bone biocomposites.
Download Full Article (PDF)
Keywords
Bioceramics, wollastonite, hydroxyapatite, precipitation, electron resonance
References
1 Meseguer-Olmo, L., Bernabeu-Esclapez, A., Ros-Martinez, E., Sánchez-Salcedo, S., Padilla, S., Martín, A.I, Vallet-Regí, M., Clavel-Sainz, M., Lopez-Prats, F., Meseguer-Ortiz, C.L.: In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO2-CaO-P2O5 system, Acta Biomater., 4, 1104 – 1113, (2008).
2 Shirazia, F.S., Mehralia, M., Oshkoura, A.A., Metselaara, H.S.C., Kadrib, N.A., AbuOsman, N.A.: Mechanical and physical properties of calcium silicate/alumina composite for biomedical engineering applications, J. Mech. Behav. Biomed., 30, 168 – 175, (2014).
3 Magallanes-Perdomo, M., Pena, P., De Aza, P.N., Carrodeguas, R.G., Rodríguez, M.A., Turrillas, X., De Aza, S., De Aza, A.H.: Devitrification studies of wollastonite-tricalcium phosphate eutectic glass, Acta Biomater., 5, 3057 – 3066, (2009).
4 Seryotkin, Y.V., Sokol, E.V., Kokh, S.N.: Natural pseudowollastonite: crystal structure, associated minerals, and geological context, Lithos, 134 – 135, (2012).
5 Zhang, N., Molenda, J.A., Fournelle, J.H., Murphy, W.L., Sahai, N.: Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells, Biomaterials, 31, 7653 – 7665, (2010).
6 Magallanes-Perdomo, M., De Aza, A.H., Mateus, A.Y., Teixeira, S., Monteiro, F.J., Aza, S., Pena, P.: In vitro study of the proliferation and growth of human bone marrow cells on apatite-wollastonite-2M glass ceramics, Acta Biomater., 6, 2254 – 2263, (2010).
7 Ballarre, J., Seltzer, R., Mendoza, E., Orellano, J.C., Mai, Y.W, García, C., Ceré, S.M.: Morphologic and nanomechanical characterization of bone tissue growth around bioactive sol-gel coatings containing wollastonite particles applied on stainless steel implants, Mater. Sci. Eng.:C, 31, 545 – 552, (2011).
8 Aza, P.N., Guitian, F., de Aza, S.: Bioactivity of wollastonite ceramics: In vitro evaluation, Scripta Metal. Mater., 31, 1001 – 1005, (1994).
9 Liu, X., Ding, C.: Morphology of apatite formed on surface of wollastonite coating soaked in simulate body fluid, Mater. Lett., 57, 652 – 655, (2002).
10 De Aza, P.N., Luklinska, Z.B., Anseau, M.R., Guitian, F., De Aza, S.: Bioactivity of pseudowollastonite in human saliva, J. Dent., 27, 107 – 113, (1999).
11 De Aza, P.N., Fernández-Pradas, J.M., Serra, P.: In vitro bioactivity of laser ablation pseudowollastonite coating, Biomaterials, 25, 1983 – 1990, (2004).
12 Habibovic, P., Barralet, J.E.: Bioinorganics and biomaterials: bone repair, Acta Biomater., 7, 3013 – 3026, (2011).
13 Carlisle, E.M.: Silicon: a possible factor in bone calcification, Science, 167, 279 – 80, (1970).
14 Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W.: Effect of sintered silicate-substituted hydroxyapatite on remodelling processes at the bone-implant interface, Biomaterials, 25, 3303 – 3314, (2004).
15 Kokubo, T.: Surface chemistry of bioactive glass-ceramics, J. Non-Cryst. Solids, 120, 138 – 151, (1990).
16 Milovac, D., Ferrer, G.G., Ivankovic, M., Ivankovic, H.: PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: morphology, mechanical properties and bioactivity, Mater. Sci. Eng.: C, 34, 437 – 445, (2014).
17 Norouzi, M., Garekani, A.A.: Corrosion protection by zirconia-based thin films deposited by a sol-gel spin coating method, Ceram. Int., 40, 2857 – 2861, (2014).
18 Sainz, M.A., Pena, P., Serena, S., Caballero, A.: Influence of design on bioactivity of novel CaSiO3-CaMg(SiO3)2 bioceramics: In vitro simulated body fluid test and thermodynamic simulation, Acta Biomater., 6, 2797 – 2807, (2010).
19 Torkittikul, P., Chaipanich, A.: Optimization of calcium chloride content on bioactivity and mechanical properties of white portland cement, Mater. Sci. Eng. C, 32, 282 – 289, (2012).
20 Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.E.(eds.): Biomaterials Science: An Introduction to materials in medicine. 2nd edition. Elsevier Academic Press, California, 2004.
21 Liu, X., Ding, C., Chu, P.K.: Mechanism of apatite formation on wollastonite coatings in simulated body fluids, Biomaterials, 25, 1755 – 1761, (2004).
22 Sahai, N., Anseau, M.: Cyclic silicate active site and stereochemical match for apatite nucleation on pseudowollastonite bioceramic-bone interfaces, Biomaterials, 26, 763 – 5770, (2005).
23 Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T.: Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W, J. Biomed. Mater. Res., 24, 721 – 734, (1990).
24 Kusrini, E., Sontang, M.: Characterization of x-ray diffraction and electron spin resonance: effects of sintering time and temperature on bovine hydroxyapatite, Radiat. Phys. Chem., 81, 118 – 125, (2012).
25 Akram, K., Ahn, J., Kwon, J.: Characterization and identification of gamma-irradiated sauces by electron spin resonance spectroscopy using different sample pretreatments, Food Chem., 138, 1878 – 1883, (2013).
26 Pattanayak, D.K., Prasad, R.C., Rao, B.T., Mohan, T.R.: Apatite wollastonite-titanium biocomposites: synthesis and in vitro evaluation, J. Am. Ceram. Soc., 89, 2172 – 2176, (2006).
27 Parida, S.K., Dash, S., Patel, S., Mishra, B.K.: Adsorption of organic molecules on silica surface, Adv. Colloid. Interfac., 121, 77 – 110, (2006).
28 Xue, W., Bandyopadhyay, A., Bose, S.: Mesoporous calcium silicate for controlled release of bovine serum albumin protein, Acta Biomater., 5, 1686 – 1696, (2009).
29 Palard, M., Champion, E., Foucaud, S.: Synthesis of silicated hydroxyapatite Ca10(PO4)6-x(SiO4)x(OH)2-x, J. Solid State Chem., 181, 1950 – 1960, (2008).
30 Shkrob, I.A., Tadjikov, B.M., Trifunac, A.D.: Magnetic resonance studies on radiation-induced point defects in mixed oxide glasses. II. spin centers in alkali silicate glasses, J. Non-Cryst. Solids, 262, 35 – 65, (2000).
31 Issa, M.A., Hassib, A.M., Dughaish, Z.H.: A study of the BaTiO,: CeO, crystal structure by EPR, J. Phys. D: Appl. Phys., 1, 2037 – 2046, (1984).
32 Park, J.: Bioceramics: Properties, characterizations, and applications. New York, Springer, 2008.
33 Hench, L.L., Wilson, J.: An Introduction to Bioceramics, Singapore. World Scientific, 1993.
34 Rokhmistrov, D.V., Nikolov, O.T., Gorobchenko, O.A., Loza, K.I.: Study of structure of calcium phosphate materials by means of electron spin resonance, Appl. Radiat. Isotopes, 70, 2621 – 2626, (2012).
35 Pietak, A.M., Reid, J.W., Sayer, M.: Electron spin resonance in silicon substituted apatite and tricalcium phosphate, Biomaterials, 26, 3819 – 3830, (2005).
36 Matković, I., Maltar-Strmečki, N., Babić-Ivančić, V., Sikirić, M.D., Noethig-Laslo, V.: Characterisation of β-tricalcium phosphate-based bone substitute materials by electron paramagnetic resonance spectroscopy, Radiat. Phys. Chem., 81, 1621 – 1628, (2012)
37 Boccaccini, A.R., Gough, J.E.: Tissue engineering using ceramics and polymers. Woodhead Publishing Limited, Cambridge, England, 2007.
Copyright
Göller Verlag GmbH